P-128 Morphokinetics and blastocyst biomarkers of twin and triplet monozygotic pregnancy using artificial intelligence

Abstract Study question Can AI reduce the rate of twin and triplet monozygotic pregnancies following elective single embryo transfer (eSET)? Summary answer can automatically annotate morphokinetic developments other biomarkers. Embryos leading multiple have slower cell divisions larger ICM than monoamniotic embryos. What is known already Monozygotic (MZT) (MTP) are a rare phenomenon in spontaneous pregnancies, but incidence increases significantly when achieved by assisted reproductive technology (ART); 0.4% vs. 1.56% MZTs, 0.004% 0.048% MTPs. It unclear what mechanisms cause an to split into two or three, although several been proposed such as culture blastocyst, decompacting ICMs, frozen-warmed transfers, hatching even ICSI. In order study this phenomenon, time-lapse imaging has used discover any signs division. design, size, duration This retrospective assessment total 8 embryos from 2018 2022 that led pregnancy (n = 4), 3) triple 1) fresh transfer. All were inseminated using ICSI technique was performed before Participants/materials, setting, methods Using CHLOE (Fairtility, Tel Aviv) we assessed morphokinetics, blastocyst biomarkers scores. Singletons compared terms morphokinetics (t-test) surface area ICM, diameter, area/embryo ratio, shape quality score ANOVA test. Main results role chance had development singletons (single vs multiple: t2: 22.1+-2 25.2+-2, p 0.04; t3: 32.56+-2.65 36.98 +-1.48, 0.01; t5: 44.09+-4.84 50.37+−0.69, 0.02; t6: 47.26+-3.87 53.59+-2.40, t7: 48.76+-4.04 55.15+-3, t8: 50.18+-4.27 57.55+-1.5, 0.008; t9: 63.27+-3.76 73.04+-5.33, 0.03). triplets twins which were, turn, triplets: t4: 33.86+-3.41 37.69+-1.61 48.95, 0.007; 58.07+-1.29 55.96, 71.23+-4.81 78.44, 0.01). ratio 0.14+−0.08 0.27+−0.06, 0.04) smaller diameter 177.2+-16.5 138.15+-8.25, 0.003). We didn’t find statistically significant differences between groups EQ 0.97 0.76, 0.36), Blast 0.87+−0.06 0.78+−0.13, 0.26), Rank, Trophectoderm quality, Area 3629+-1361 4151 +- 569, 0.48) 1.64+−0.45 1.27+−0.12, 0.15). Limitations, reasons for caution The main limitation number cases included study, studied one triplet, 3 4 pregnancies. Wider implications findings might be first time analyse behaviour resulting slowly dividing and/or with large could result pregnancy. Trial registration not applicable